Media Required:

• To grow the patches seen in the photographs, you will need YED plates.
 recipe for YED plates
• Sterile, empty petri plates and media ingredients are available from many sources, including:
 VWR/SP Scientific Products
 
• To grow the strain before making the patches, you will need selective medium. This is synthetic complete medium lacking leucine (SC-leu). Pre-mixed ingredients for SC-LEU medium can be purchased from  Bio 101 .
• Pre-poured plates are also available from  Bio 101

Yeast Strain
The yeast strain is genotype: a ade2 ade3 ura3 leu2 lys2 (pDE255 ADE3 LEU2) and is available from the  ATCC  as accession number 201926 Saccharomyces cerevisiae. Soon, it may be available from  Carolina Biological Supply

Tecnhniques

General Yeast handling techniques can be found though the  GENE project  Yeast pages.

Preparing the patch

1. Two weeks before the lab, streak the strain on an SC-LEU plate.
2. Incubate at room temp for 1 week (5 days at 30C)
3. resuspend one or two large colonies (the amount is not critical - a 2mm cube should be sufficient) into 1ml sterile water.
4. Using a sterile Q-tip dipped in the suspension, spread a roughly 2cm x 2cm patch onto several YED plates.
5. Let these grow 1 week at room temp (or 5 days at 30C)

Student experiments
Anything goes. We use sterile pointed toothpicks so that they can take small samples. Plates should grow for 1 week at room temp or 5 days at 30C.